Genetic laboratory

We have a team of specialists dedicated to fulfilling our mission
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AMS Laborator Genetic has invested and is constantly investing in consolidating its medical infrastructure. Thus, the company currently has high-performance laboratory equipment which is grouped within the two cores of its analysis activity:

  • Genetic laboratory
  • Molecular diagnostic laboratory

The genetics lab was started and developed as a project in partnership with the American company Natera and resulted in the implementation of the non-invasive prenatal screening test Panorama.

The laboratory uses the most modern genetic technologies in the world, its infrastructure being identical to that of Natera laboratories. At the same time, the specialists who perform the analyzes, together with the specialized competence in the field, followed specific training programs.

The laboratory has all the necessary technologies and is also certified for performing NGS – Next Generation Sequencing tests. The applications of NGS technology are very wide, and in our laboratory can be implemented genetic profiling analyzes in common cancers, in rare diseases, as well as analyzes for the identification of molecular targets associated with oncological therapies.

At the same time, the existence of an adequate and well-equipped space is crucial for the good development of the analytical flow and the obtaining of correct results

Thus, in order to eliminate any risk of accidental contamination, the organization of the laboratory is based on the spatial separation of the various methodological steps and on ensuring a one-way analytical flow. The working methodology was verified and validated by Natera specialists. (see validation certificates here and here)

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  1. Sample reception room – Space for reception of the samples (from partner clinics) to be analyzed
  2. Biosecurity room – The activities done here are: plasma isolation, DNA extraction and purification, storage of the extracted DNA.
  3. Pre-PCR room – Library preparation by DNA fragmentation and addition of specific oligonucleotide sequences and in vitro enzymatic amplification by PCR.
  4. Post-PCR 1 room – Libraries purification and their verification thorough automatic electrophoresis.
  5. Post-PCR 2 room – DNA amplification (PCR reaction) and sequencing (two Illumina sequencing equipment, NextSeq 500 and NextSeq 550).

The molecular diagnostic laboratory allows real-time PCR testing. They are used to test for the presence of SARS-CoV-2 infection, as well as for other tests such as Bladder EpiCheck (to detect the recurrence of bladder tumors), the presence of human papilloma virus (HPV) or thrombophilic profile.

The laboratory complies with all regulatory requirements for laboratories with a medium to high risk of contamination of staff and consists of 7 rooms:

  1. Space for the personnel to equipping with protective equipment;
  2. Sample reception room – space for the reception and decontamination of samples to be analyzed;
  3. Extraction chamber – space for the extraction of nucleic acids from biological samples (nasopharyngeal exudate, saliva, bronchial aspirate and others) in order to obtain DNA or RNA;
  4. prePCR chamber – space for the preparation of the reaction mixture to be used in the step of amplification of nucleic acids;
  5. PCR chamber – space for amplifying and purifying amplicon libraries in order to prepare samples for the amplification and detection stage of test-specific targets;
  6. Decontamination/unequipping room – space for decontamination and unequipping the protective equipment of the personnel;
  7. Waste room – space for the storage of waste resulting from the processing of samples and those resulting from the protective equipment of personnel.

The laboratory is equipped with all the necessary equipment to perform the RT-PCR type test:

  • Automated extractors;
  • Biosecurity hoods;
  • Specific RT-PCR equipments.